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- 抗原 See all ERAS 抗体
- ERAS (ES cell expressed Ras (ERAS))
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抗原表位
- AA 13-42, N-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ERAS antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- 纯化方法
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
- 免疫原
- This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human ERAS.
- 亚型
- Ig Fraction
- Top Product
- Discover our top product ERAS Primary Antibody
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anti-ES cell expressed Ras (ERAS) antibody
ERAS 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (C-Term) antibodyERAS 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 19-52), (N-Term) antibodyERAS 适用: 小鼠 WB, FACS 宿主: 兔 Polyclonal RB51538 unconjugated
anti-ES cell expressed Ras (ERAS) (AA 202-236), (C-Term) antibodyERAS 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal RB52380 unconjugated
anti-ES cell expressed Ras (ERAS) (N-Term) antibodyERAS 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 3-230) antibodyERAS 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) (AA 1-233) antibodyERAS 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-ES cell expressed Ras (ERAS) antibody (Biotin)ERAS 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal Biotin
anti-ES cell expressed Ras (ERAS) antibody (FITC)ERAS 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
anti-ES cell expressed Ras (ERAS) antibody (HRP)ERAS 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal HRP
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- 应用备注
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For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2 mg/mL
- 缓冲液
- Supplied in PBS with 0.09 % (W/V) sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- 抗原
- ERAS (ES cell expressed Ras (ERAS))
- 别名
- ERAS (ERAS 产品)
- 别名
- HRAS2 antibody, HRASP antibody, HRasp antibody, Ha-Ras2 antibody, ecat5 antibody, ES cell expressed Ras antibody, ES cell-expressed Ras antibody, ERAS antibody, Eras antibody
- 背景
- Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.
- 分子量
- 25 kDa
- 基因ID
- 3266
- UniProt
- Q7Z444
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