Phenylalanine Hydroxylase 抗体 (AA 71-208)
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- 抗原 See all Phenylalanine Hydroxylase 抗体
- Phenylalanine Hydroxylase
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抗原表位
- AA 71-208
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Phenylalanine Hydroxylase antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Phenylalanine-4-hydroxylase(PAH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Phenylalanine-4-hydroxylase(PAH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: phenylalanine hydroxylase
Protein Name: Phenylalanine-4-hydroxylase - 纯化方法
- Immunogen affinity purified.
- 免疫原
- E. coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1% and 88.4% amino acid (aa) sequence identity with mouse and rat PAH, respectively.
- 亚型
- IgG
- Top Product
- Discover our top product Phenylalanine Hydroxylase Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- Phenylalanine Hydroxylase
- 别名
- PAH (Phenylalanine Hydroxylase 产品)
- 别名
- cb877 antibody, wu:fb02f10 antibody, zgc:65981 antibody, GB18494 antibody, PAH antibody, pku antibody, pku1 antibody, DDBDRAFT_0206188 antibody, DDBDRAFT_0231664 antibody, DDB_0206188 antibody, DDB_0231664 antibody, AW106920 antibody, PH antibody, PKU antibody, PKU1 antibody, phenylalanine-4-hydroxylase antibody, phenylalanine hydroxylase antibody, protein henna antibody, phenylalanine 4-monooxygenase antibody, pah antibody, LOC408622 antibody, PAH antibody, CpipJ_CPIJ002149 antibody, Pah antibody
- 物质类
- Chemical
- 背景
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Phenylalanine hydroxylase (PAH) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.
Synonyms: Phenylalanine-4-hydroxylase, PAH, Phe-4-monooxygenase - 基因ID
- 5053
- UniProt
- P00439
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