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EGF 抗体 (AA 974-1026)

Name: EGF 抗体 (AA 974-1026)
SKU: ABIN5518908
Availability: OutOfStock

EGF 适用: 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

Western blot analysis of EGF using anti-EGF antibody . (EGF 抗体 (AA 974-1026))

IHC analysis of EGF using anti-EGF antibody . (EGF 抗体 (AA 974-1026))

IHC

3 images

(2 references)

产品编号 ABIN5518908

发货至: 中国

Datasheet as PDF

抗原 See all EGF 抗体

EGF (Epidermal Growth Factor (EGF))
抗原表位
45

16

10

7

7

7

7

7

6

6

6

5

5

4

4

4

4

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 974-1026
适用
153

88

46

12

11

5

4

1

1
大鼠
宿主
215

31

10

2

2

1
兔
Compatible Secondaries
克隆类型
221

39
多克隆
标记
111

34

20

16

11

7

4

4

4

4

4

4

4

4

4

4

4

2

2

2

2

2

2

2

1

1

1

1
This EGF antibody is un-conjugated
应用范围
200

91

63

53

53

28

25

23

16

16

15

13

10

8

4

4

3

3

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(Egf) detection. Tested with WB, IHC-P, ELISA in Rat.

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for Pro-epidermal growth factor(Egf) detection. Tested with WB, IHC-P, ELISA in Rat.
Gene Name: epidermal growth factor
Protein Name: Pro-epidermal growth factor

纯化方法

Immunogen affinity purified.

免疫原

E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.

亚型

IgG

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anti-Epidermal Growth Factor (EGF) antibody
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anti-Epidermal Growth Factor (EGF) (AA 31-53) antibody
EGF 适用: 人 ELISA, IHC (p), IF (p), IF (cc), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Epidermal Growth Factor (EGF) (AA 1-30) antibody
EGF 适用: 小鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Wang, Li, Fu, Li, Yang, Zhang, Zhu, Yang, Gu, Xing, Zhang: "Exemestane Attenuates Hepatic Fibrosis in Rats by Inhibiting Activation of Hepatic Stellate Cells and Promoting the Secretion of Interleukin 10." in: Journal of immunology research, Vol. 2017, pp. 3072745, (2018) (PubMed).

Messini: "[Therapy of total arrhythmia due to atrial fibrillation and flutter]." in: La Clinica terapeutica, Vol. 57, Issue 2, pp. 109-28, (1971) (PubMed).
抗原

EGF (Epidermal Growth Factor (EGF))

别名

Egf (EGF 产品)

别名

HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody

背景

EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Synonyms: Pro-epidermal growth factor, EGF, Epidermal growth factor, Egf

基因ID

25313

UniProt

P07522

途径

NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation