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Choline Acetyltransferase 抗体 (AA 446-652)

Name: Choline Acetyltransferase 抗体 (AA 446-652)
SKU: ABIN5518818
Availability: OutOfStock

CHAT 适用: 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

Western blot analysis of CHAT using anti- CHAT antibody . (Choline Acetyltransferase 抗体 (AA 446-652))

IHC analysis of CHAT using anti- CHAT antibody . (Choline Acetyltransferase 抗体 (AA 446-652))

IHC

3 images

(7 references)

产品编号 ABIN5518818

发货至: 中国

Datasheet as PDF

抗原 See all Choline Acetyltransferase (CHAT) 抗体

Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
抗原表位
16

15

6

6

2

2

2

2

1

1

1

1
AA 446-652
适用
71

46

41

17

5

2

2

1

1
小鼠, 大鼠
宿主
65

5

5

4

3
兔
Compatible Secondaries
克隆类型
70

12
多克隆
标记
40

4

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Choline Acetyltransferase antibody is un-conjugated
应用范围
42

22

22

20

16

14

13

13

6

5

4

3

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Rabbit IgG polyclonal antibody for Choline O-acetyltransferase(CHAT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

交叉反应（详细）

Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

产品特性

Rabbit IgG polyclonal antibody for Choline O-acetyltransferase(CHAT) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: choline O-acetyltransferase
Protein Name: Choline O-acetyltransferase

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively.

亚型

IgG

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anti-Choline O-Acetyltransferase (CHAT) (C-Term) antibody
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anti-Choline O-Acetyltransferase (CHAT) antibody
Microarray verified CHAT 适用: 人 IHC 宿主: 小鼠 Monoclonal MAB872084 unconjugated

anti-Choline O-Acetyltransferase (CHAT) (C-Term) antibody
CHAT 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Choline O-Acetyltransferase (CHAT) (AA 331-630) antibody
CHAT 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Yu, Xia, Tang, Xu, Wei, Chen, Dai, Gong, Bi: "Acetylcholine acts through M3 muscarinic receptor to activate the EGFR signaling and promotes gastric cancer cell proliferation." in: Scientific reports, Vol. 7, pp. 40802, (2018) (PubMed).

Zhao, Xu, Pan, Zhu, Fu, Guo, Lu, Wang: "Pharmacologic activation of cholinergic alpha7 nicotinic receptors mitigates depressive-like behavior in a mouse model of chronic stress." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 234, (2018) (PubMed).

Hu, Wang, Ren, Liu: "Autonomic remodeling may be responsible for decreased incidence of aortic dissection in STZ-induced diabetic rats via down-regulation of matrix metalloprotease 2." in: BMC cardiovascular disorders, Vol. 16, Issue 1, pp. 200, (2017) (PubMed).

Wang, He, Zhong, Lv, Xiao, Liu: "Overexpression of NTRK1 Promotes Differentiation of Neural Stem Cells into Cholinergic Neurons." in: BioMed research international, Vol. 2015, pp. 857202, (2016) (PubMed).

Liu, Deng, Zhang, Zhang: "Wnt3a expression during the differentiation of adipose-derived stem cells into cholinergic neurons." in: Neural regeneration research, Vol. 7, Issue 19, pp. 1463-8, (2015) (PubMed).

Zhang, Li, Gu, Liu, Xu, Cui, Sun: "Thalidomide influences growth and vasculogenic mimicry channel formation in melanoma." in: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 60, (2009) (PubMed).

Zhang, Qi, Li, Zhang, Xu, Wang, Sun: "Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 62, (2009) (PubMed).
抗原

Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

别名

CHAT (CHAT 产品)

别名

CHOACTASE antibody, CMS1A antibody, CMS1A2 antibody, ChoAcTase antibody, B230380D24Rik antibody, choline O-acetyltransferase antibody, choline O-acetyltransferase a antibody, choline acetyltransferase antibody, carnitine O-acetyltransferase antibody, CHAT antibody, chata antibody, Chat antibody, PGTG_00551 antibody, LOC100533322 antibody

背景

Choline acetyltransferase (commonly abbreviated as ChAT, but sometimes CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans, the choline acetyltransferase enzyme is encoded by the CHAT gene. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.

Synonyms: ChAT | CHOACTase | Choline acetylase | choline acetyltransferase | Choline O acetyltransferase | CMS1A | CMS1A2 | CMS6 | P28329

基因ID

1103

UniProt

P28329

途径

Skeletal Muscle Fiber Development