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GAPDH 抗体 (AA 136-335)

Name: GAPDH 抗体 (AA 136-335)
SKU: ABIN5518757
Availability: OutOfStock

GAPDH 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

Western blot analysis of GAPDH expression in rat brain extract ( Lane 1), rat kidney extract ( Lane 2), mouse kidney extract ( Lane 3), HELA whole cell lysates ( Lane 4) and MCF-7 whole cell lysates( Lane 5). (GAPDH 抗体 (AA 136-335))

GAPDH was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- GAPDH Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL. (GAPDH 抗体 (AA 136-335))

IHC

GAPDH was detected in paraffin-embedded sections of human thyroid cancer tissues using rabbit anti- GAPDH Antigen Affinity purified polyclonal antibody (Catalog # ) at 1 µg/mL. (GAPDH 抗体 (AA 136-335))

IHC

3 images

(115 references)

产品编号 ABIN5518757

发货至: 中国

Datasheet as PDF

抗原 See all GAPDH 抗体

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
抗原表位
34

22

13

11

7

6

6

5

4

4

3

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 136-335
适用
212

144

137

70

64

52

36

27

22

20

19

19

17

16

11

11

11

9

9

8

4

4

4

4

4

3

3

2

2

2

2

2

1

1

1

1

1
人, 小鼠, 大鼠
宿主
159

94

17

11

4
兔
Compatible Secondaries
克隆类型
186

97
多克隆
标记
153

33

18

17

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1
This GAPDH antibody is un-conjugated
应用范围
228

96

71

65

43

37

30

24

16

16

15

14

7

6

5

4

4

3

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Rabbit IgG polyclonal antibody for Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glyceraldehyde-3-phosphate dehydrogenase
Protein Name: Glyceraldehyde-3-phosphate dehydrogenase

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human GAPDH recombinant protein (Position: N136-E335). Human GAPDH shares 95% and 94.5% amino acid (aa) sequence identity with mouse and rat GAPDH, respectively.

亚型

IgG

Top Product

Discover our top product GAPDH Primary Antibody
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH 适用: 人, 小鼠, 大鼠, 兔, Pig, 小鸡, Cow, Various Species WB, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH 适用: 人, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1A10A10 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibody
GAPDH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 154-324) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-148) antibody
GAPDH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Wang, Xue, Zhang, Bu, Zhu, Lai: "Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 10, pp. 1376-85, (2018) (PubMed).

Duan, Man, Tang, Yao, Tao, Yu, Liang, Makawana, Zou, Wang, Zhu: "Design, Synthesis and Antitumor Activity of Novel link-bridge and B-Ring Modified Combretastatin A-4 (CA-4) Analogues as Potent Antitubulin Agents." in: Scientific reports, Vol. 6, pp. 25387, (2018) (PubMed).

Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, (2018) (PubMed).

Zou, Duan, Wang, Gao, Chen, Ou, Liang, Wang, Yuan, Wang, Zhu: "DYT-40, a novel synthetic 2-styryl-5-nitroimidazole derivative, blocks malignant glioblastoma growth and invasion by inhibiting AEG-1 and NF-κB signaling pathways." in: Scientific reports, Vol. 6, pp. 27331, (2018) (PubMed).

Guo, Li, Qian, Bi, He, Jin, Luo, Li, Meng, Li: "TGEV infection up-regulates FcRn expression via activation of NF-κB signaling." in: Scientific reports, Vol. 6, pp. 32154, (2018) (PubMed).

Li, Chen, Xiao, Li, Wang, Su, Guo, Liu, Yan, Yang, Zhang, Bu: "Mouse Sirt3 promotes autophagy in AngII-induced myocardial hypertrophy through the deacetylation of FoxO1." in: Oncotarget, Vol. 7, Issue 52, pp. 86648-86659, (2018) (PubMed).

Feng, Zhang, Zhou, Zhou, Li, Guo, Feng, Ma, Huang, Huang: "mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27." in: Oncotarget, Vol. 8, Issue 4, pp. 6304-6318, (2018) (PubMed).

Li, Guan, Liu, Zhao, Li, Zhang, Tan, Mi, Li: "Epoxyeicosanoids prevent intervertebral disc degeneration in vitro and in vivo." in: Oncotarget, Vol. 8, Issue 3, pp. 3781-3797, (2018) (PubMed).

Hu, Cao, Pang, Luo, Zou, Feng, Li, Chen, Huang: "Tumor grade related expression of neuroglobin is negatively regulated by PPARγ and confers antioxidant activity in glioma progression." in: Redox biology, Vol. 12, pp. 682-689, (2018) (PubMed).

Liao, Xing, Xu, Liu, Zhang: "Evaluation of the circulating level of fibroblast activation protein α for diagnosis of esophageal squamous cell carcinoma." in: Oncotarget, Vol. 8, Issue 18, pp. 30050-30062, (2018) (PubMed).

Cai, Tan, Li, Gao, Li, Li, Tang, Li, Hui: "Glucagon-Like Peptide-1 (GLP-1) Treatment Ameliorates Cognitive Impairment by Attenuating Arc Expression in Type 2 Diabetic Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 4334-4342, (2018) (PubMed).

Wu, Sun, Ye, Zhang, Guo, Huang, Jing, Xiang, Yu, Guo: "Iguratimod prevents ovariectomy‑induced bone loss and suppresses osteoclastogenesis via inhibition of peroxisome proliferator‑activated receptor‑γ." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8200-8208, (2018) (PubMed).

Liu, Liu, Tan, Tang, Long, Li, Liang, Chu, Yang: "Gaseous signalling molecule SO2 via Hippo‑MST pathway to improve myocardial fibrosis of diabetic rats." in: Molecular medicine reports, Vol. 16, Issue 6, pp. 8953-8963, (2018) (PubMed).

Wu, Xing, Zhang, Mao, Chen, Liang, Wang, Ren, Cui, Jiang, Wang, Zou, Ji: "Autophagy promotes fibrosis and apoptosis in the peritoneum during long-term peritoneal dialysis." in: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 1190-1201, (2018) (PubMed).

Gu, Fang, Kang, Hu, Yu, Li, Cheng, Gao: "Effect of ALDH2 on High Glucose-Induced Cardiac Fibroblast Oxidative Stress, Apoptosis, and Fibrosis." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 9257967, (2018) (PubMed).

Li, Wang, Qin, Fan, Tian, Liu: "Fingolimod suppresses neuronal autophagy through the mTOR/p70S6K pathway and alleviates ischemic brain damage in mice." in: PLoS ONE, Vol. 12, Issue 11, pp. e0188748, (2018) (PubMed).

Zou, Zhang, Yuan, Yang, Wu, Song, Zhu, Mao, Chen: "Endogenous hormone 2-methoxyestradiol suppresses venous hypertension-induced angiogenesis through up- and down-regulating p53 and id-1." in: Journal of cellular and molecular medicine, Vol. 22, Issue 2, pp. 957-967, (2018) (PubMed).

Huang, Pan, Mao, Zhang, Ma, Xi, You: "Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes." in: Inflammation, Vol. 41, Issue 2, pp. 562-568, (2018) (PubMed).

Miao, Margolis, Gao, Voss, Li, Martini, Norton, Bossé, Wankowicz, Cullen, Horak, Wind-Rotolo, Tracy, Giannakis, Hodi, Drake, Ball, Allaf, Snyder, Hellmann, Ho, Motzer, Signoretti, Kaelin, Choueiri et al.: "Genomic correlates of response to immune checkpoint therapies in clear cell renal cell carcinoma. ..." in: Science (New York, N.Y.), Vol. 359, Issue 6377, pp. 801-806, (2018) (PubMed).

Yang, Zhang, Li, Wen: "MALAT1 enhanced the proliferation of human osteoblasts treated with ultra‑high molecular weight polyethylene by targeting VEGF via miR‑22‑5p." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1536-1546, (2018) (PubMed).

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抗原

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

别名

GAPDH (GAPDH 产品)

别名

G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

背景

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37 kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. GAPDH is mapped to 12p13.31. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus.

Synonyms: EC 1.2.1.12 | EC1.2.1.12 | G3P | GAPD | GAPDH | P04406

基因ID

2597

UniProt

P04406