beta Amyloid 抗体 (C-Term)
(4 references)
(2 validations)-
- 抗原 See all beta Amyloid (Abeta) 抗体
- beta Amyloid (Abeta) (Amyloid beta (Abeta))
-
抗原表位
- C-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This beta Amyloid antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 特异性
- A-beta 1-40, A-beta 1-42, A-beta 1-43 and APP
- 交叉反应 （详细）
- Human, mouse, rat (other species not tested)
- 纯化方法
- Protein G affinity purification
- 免疫原
- Synthetic peptide derived from Human Abeta 1-42 protein
- 克隆位点
- 9E8-1A3
- 亚型
- IgG1
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anti-Amyloid beta (Abeta) (N-Term) antibody
Abeta 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) (AA 711-760) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) antibodyAbeta 适用: 人 WB, ELISA, IHC, IP, IEM 宿主: 兔 Chimeric 6E10 unconjugated Recombinant Antibody
anti-Amyloid beta (Abeta) (Ser15) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) (pThr743) antibodyAbeta 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Amyloid beta (Abeta) antibodyAbeta 适用: 人 WB, IHC, IF, ICC 宿主: 小鼠 Monoclonal AB9 unconjugated
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- 应用备注
- ELISA: 1:40,000, WB: 1:4,000, IF: 1:2,000
- 限制
- 仅限研究用
-
- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104425
- 日期
- 2022.05.12
- 抗原
- beta Amyloid
- Lot Number
- 190522A
- Method validated
- Immunohistochemistry
- Positive Control
Adult (24 months) mouse brain
3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde
- Negative Control
We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.
- Notes
The beta amyloid antibody (Amyloid beta) (C-Term) ABIN5508784 works in IHC-P, especially at higher concentrations (1:200 and 1:500), and without the use of any antigen retrieval treatment.
- Primary Antibody
- ABIN5508784
- Secondary Antibody
- goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)
- Full Protocol
- Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
- Wash, dehydrate, and embed samples in paraffin wax.
- Wash several times with 0.01 M PBS.
- Cut intestines and brain with a microtome into 6µm sections and mount on glass slides.
- After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
- Incubate sections with primary anti-beta amyloid (C-Term) (antibodies Online, ABIN5508784, lot 190522A) diluted 1:200, 1:500, 1:1000, and 1:2000 in PBS-BSA-PLL ON at RT.
- Wash sections 3x 5 min with 0.01 M PBS.
- Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1 M PBS for 1 h at RT.
- Wash sections 3x 5 min with 0.01M PBS.
- Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
- Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.
- Experimental Notes
Antigen retrieval treatment was also tested. In this case, sections were processed for microwave antigen retrieval for 10 min(95-100 °C) in 10 mM sodium citrate buffer (pH 6.0). After 20 min of spontaneous cooling, sections were washed twice for 5 min with distilled water and for 5 min with PBS.
生效 #104425 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Staining of beta-amyloid positive cells in the adult mouse brain using ABIN5508784 at 40x magnification.]()
Staining of beta-amyloid positive cells in the adult mouse brain using ABIN5508784 at 40x magnification.
Full Methods - by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104493
- 日期
- 2023.03.15
- 抗原
- Abeta
- Lot Number
- 190522A
- Method validated
- Immunohistochemistry
- Positive Control
3-month-old Taconic mouse Model 1349 brain fixed in 4% paraformaldehyde
- Negative Control
One control slice for each experimental procedure processed omitting the primary antibody; overnight incubation in diluent solution only.
- Notes
The beta Amyloid antibody (Amyloid beta) (C-Term) ABIN5508784 works in IHC-P, especially at higher concentrations (1:200 and 1:500). Tyramide amplification strongly enhances the positive signal.
- Primary Antibody
- ABIN5508784
- Secondary Antibody
- poly-HRP conjugated goat anti-rabbit antibody
- Full Protocol
- Perfuse mice with paraformaldehyde 4% in 0.1 M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2 h at RT.
- Wash, dehydrate, and embed samples in paraffin wax.
- Wash several times with 0.01 M PBS.
- Cut intestines and brain with a microtome into 6 µm sections and mount on glass slides.
- After paraffin removal, incubate sections for 1 h at RT in PBS containing 1% albumin from chicken egg white (Sigma, A5378) and 0.3% Triton-X-100 (BioRad, 161-0407, lot 00583) to block non-specific binding sites.
- Incubate sections with primary anti-beta amyloid (N-Term) (antibodies-online, ABIN5508784, lot 190522A) diluted 1:200, 1:500, 1:1000, and 1:2000 in 0.1 M PBS-BSA-PLL ON at RT.
- Wash sections 3x 5 min with 0.01 M PBS.
- Incubate sections with poly-HRP conjugated goat anti-rabbit antibody for 1 h at RT.
- Wash sections 3x 5 min with 0.01 M PBS.
- Incubate sections Tyramide working solution (for 5 sections: 100X Tyramide stock solution 5 μL, 100X H2O2 solution 5 μL, and 1X Reaction buffer 500 μL; Thermo Fisher Scientific, B40915, lot 2086865) for 10 min.
- Stop the reaction with the Reaction Stop Reagent working solution.
- Wash sections 3x 5 min with 0.01M PBS.
- Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
- Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with a digital camera at 40x magnification.
- Experimental Notes
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, pH 7.4
- 储存液
- Without preservative
- 注意事项
- Avoid freezing/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C to -80°C.
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-
: "ImmunoPEGliposome-mediated reduction of blood and brain amyloid levels in a mouse model of Alzheimer's disease is restricted to aged animals." in: Biomaterials, Vol. 112, pp. 141-152, (2016) (PubMed).
: "Anti-Aβ-MAb and dually decorated nanoliposomes: effect of Aβ1-42 peptides on interaction with hCMEC/D3 cells." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 81, Issue 1, pp. 49-56, (2012) (PubMed).
: "Versatile and efficient targeting using a single nanoparticulate platform: application to cancer and Alzheimer's disease." in: ACS nano, Vol. 6, Issue 7, pp. 5866-79, (2012) (PubMed).
: "The binding affinity of anti-Aβ1-42 MAb-decorated nanoliposomes to Aβ1-42 peptides in vitro and to amyloid deposits in post-mortem tissue." in: Biomaterials, Vol. 32, Issue 23, pp. 5489-97, (2011) (PubMed).
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: "ImmunoPEGliposome-mediated reduction of blood and brain amyloid levels in a mouse model of Alzheimer's disease is restricted to aged animals." in: Biomaterials, Vol. 112, pp. 141-152, (2016) (PubMed).
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- 抗原
- beta Amyloid (Abeta) (Amyloid beta (Abeta))
- 别名
- Amyloid beta (Abeta 产品)
- 别名
- AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN-II antibody, PN2 antibody, aaa antibody, abeta antibody, abpp antibody, ad1 antibody, appi antibody, ctfgamma antibody, cvap antibody, pn2 antibody, Abeta antibody, Abpp antibody, Adap antibody, Ag antibody, Cvap antibody, E030013M08Rik antibody, betaApp antibody, amyloid beta precursor protein antibody, amyloid beta (A4) precursor protein antibody, APP antibody, app antibody, App antibody
- 背景
- Amyloid beta (Aβ or Abeta) is a peptide of 36 - 43 amino acids derived from the cleavage of APP (Amyloid Precursor Protein) by β- and γ-secretases. It is involved in Alzheimer´s disease, among other severe neurological disorders. It has been proved that the Aβ could be a crucial target for these important diseases treatment.
- 途径
- Inflammasome
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Staining of beta-amyloid positive neurons in the hippocampus and cerebral cortex of a 3-month-old transgenic mouse using ABIN5508784. Tyramide amplification. No counterstain.
