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SMT1 抗体

SMT1 适用: Arabidopsis thaliana IL, WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5326722
发货至: 中国
  • 抗原 See all SMT1 products
    SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))
    适用
    Arabidopsis thaliana
    宿主
    克隆类型
    多克隆
    应用范围
    Immunolocalization (IL), Western Blotting (WB)
    交叉反应 (详细)
    Not reactive in: Hordeum vulgare, Triticum aestivum, Withania somnifera
    预测反应
    Amborella trichopoda, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium mexicanum, Medicago truncatula, Populus trichopocarpa, Prunus persica, Ricinus communis, Theobroma cacao, Vitis vinifera,
    产品特性
    Expected / apparent Molecular Weight of the Antigene: 38 kDa
    纯化方法
    affinity purified
    免疫原
    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SMT1 Q9LM02, At5g13710
  • 应用备注
    1: 500 - 1:1 000 with ECL Advance or + (WB), 1: 50 - 1: 100 (IL)
    说明

    SMT1 antibody characterization in Western Blot and immunofluorescence labeling: Boutté Y et al. (2010). Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis. EMBO J. 29, 5465-5458.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 200 μL of sterile water.
    注意事项
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    store lyophilized/reconstituted at -20C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).

    Boutté, Frescatada-Rosa, Men, Chow, Ebine, Gustavsson, Johansson, Ueda, Moore, Jürgens, Grebe: "Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis." in: The EMBO journal, Vol. 29, Issue 3, pp. 546-58, (2010) (PubMed).

  • 抗原
    SMT1 (Cycloartenol-C-24-Methyltransferase (SMT1))
    别名
    Sterol methyltransferase 1 (SMT1) (SMT1 产品)
    别名
    CEPHALOPOD antibody, CPH antibody, MSH12.18 antibody, MSH12_18 antibody, SAM:CYCLOARTENOL-C24-METHYLTRANSFERASE antibody, sterol methyltransferase 1 antibody, sterol methyltransferase 1 antibody, SMT1 antibody
    背景
    AGI Code: At5g13710
    SMT1 (EC=2.1.1.41) is an enzyme involved in steroid biosynthesis localized specific to the Endoplasmic Reticulum (ER), Boutte et al., 2009 . It is catalyzing the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol. Alternative names: Cycloartenol-C-24-methyltransferase, 24-sterol C-methyltransferase 1, Protein STEROL METHYLTRANSFERASE 1, Protein CEPHALOPOD
    分子量
    38 kDa
    UniProt
    Q9LM02
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