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STON1 抗体 (AA 529-620)

STON1 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 1F3 unconjugated
产品编号 ABIN524426
发货至: 中国
  • 抗原 See all STON1 抗体
    STON1 (Stonin 1 (STON1))
    抗原表位
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 529-620
    适用
    • 21
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 18
    • 3
    小鼠
    克隆类型
    • 19
    • 2
    单克隆
    标记
    • 14
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This STON1 antibody is un-conjugated
    应用范围
    • 13
    • 8
    • 3
    • 2
    • 1
    • 1
    ELISA, Immunofluorescence (IF)
    原理
    Mouse monoclonal antibody raised against a partial recombinant SBLF.
    序列
    SLKSVVVVQG AYVELQAFVN MASLAQRSSY AGSLRSCDNI RIHFPVPSQW IKALWTMNLQ RQKSLKAKMN RRACLGSLQE LESEPVIQVT VG
    交叉反应
    产品特性
    Antibody Reactive Against Recombinant Protein.
    免疫原
    SBLF (NP_006864, 529 a.a. ~ 620 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
    克隆位点
    1F3
    亚型
    IgG2a
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 生效 #101553 (Immunofluorescence)
    '独立验证'标志
    by
    Lab of Prof. Dr. S.A. Kushner, Department of Psychiatry, Erasmus University Medical Center
    No.
    #101553
    日期
    2018.01.12
    抗原
    STON1
    Lot Number
    08242-1F3
    Method validated
    Immunofluorescence
    Positive Control
    U-373 cell line (human glioblastoma astrocytoma)
    Negative Control
    secondary only
    Notes

    The STON1 antibody ABIN524426 specifically labels the targeted antigen in sample in IF.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN524426
    Secondary Antibody
    donkey anti-mouse A647-conjugated antibody (Sanbio, 715-605-150)
    Full Protocol
    • Grow U-373 cells (human glioblastoma astrocytoma) in DMEM, high glucose, glutamax (Gibco, product number: 31966-021, lot number: 1894785 supplemented with Fetal Bovine Serum (Gibco, 10082-147, lot 1907451) and penicillin streptomycin (Gibco, 15140-122, lot 1910857), at 37°C and 5% CO2 in 2ml on a coverslips.
    • Transfect cells with aCSPG4-GFP expression plasmid using X-tremeGENE HP (Sigma) accoding to the manufacturer’s recommendations.
    • Fix cells on coverslips in 4% PFA for 20min at RT.
    • Wash cells 3x for 5min with PBS.
    • Wash coverslips for 5min with staining solution (50mM TrisHCl pH7.4, 154mM NaCl, 0.25% w/v gelatine, 5% v/v triton x-100).
    • Incubate cells with primary mouse anti-human STON1 antibodylavivirus (antibodies-online, ABIN524426, lot 08242-1F3) diluted 1:100 in staining solution ON at 4°C.
    • Wash cells 3x for 5min with PBS.
    • Incubate cells with secondary donkey anti-mouse A647-conjugated antibody (Sanbio, 715-605-150) diluted 1:200 in staining solution for 1.5h at RT.
    • Wash cells 2x for 5min with PBS.
    • DAPI counterstain.
    • Mount coverslips on glass slides in Mowiol 4-88 antifade reagent (Sigma, 81381, lot BCBL1789V).
    • Image acquisition with LSM 700, 63x magnification, 1024x1024 resolution.
    Experimental Notes
    • ABIN524426 was used in cells overexpressing CSPG4 fused to GFP. Stonin is expected to be abundantly expressed in the cells.

    • No signal was detected in the secondary antibody only control (not shown).

  • 缓冲液
    In 1x PBS, pH 7.4
    注意事项
    Aliquot to avoid repeated freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • 抗原
    STON1 (Stonin 1 (STON1))
    别名
    STON1 (STON1 产品)
    别名
    SALF antibody, SBLF antibody, STN1 antibody, STNB1 antibody, DKFZP469K176 antibody, 4921524J06Rik antibody, C86231 antibody, Salf antibody, Sblf antibody, stonin 1 antibody, STON1 antibody, ston1 antibody, Ston1 antibody
    背景
    Full Gene Name: stonin 1
    Synonyms: DKFZp781K2462,MGC149803,MGC149804,SBLF,STN1,STNB1,stoned-b1
    基因ID
    11037
    NCBI登录号
    NM_006873
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