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RELB 抗体 (AA 1-579)

Name: RELB 抗体 (AA 1-579)
SKU: ABIN519730
Availability: OutOfStock
Rating: 5 (1 reviews)

RELB 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 1-579) antibody (ABIN519730) (RELB 抗体 (AA 1-579))

Western Blot analysis of RELB expression in transfected 293T cell line by RELB MaxPab polyclonal antibody. (RELB 抗体 (AA 1-579))

Proximity Ligation Analysis of protein-protein interactions between RELB and RELA. (RELB 抗体 (AA 1-579))

PLA

3 images

(1 validation)

产品编号 ABIN519730

发货至: 中国

Datasheet as PDF

抗原 See all RELB 抗体

RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))
抗原表位
16

15

12

8

5

5

5

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 1-579
适用
101

72

35

12

12

8

6

6

5

3

1

1

1
人
宿主
99

6
兔
Compatible Secondaries
克隆类型
94

11
多克隆
标记
58

5

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1
This RELB antibody is un-conjugated
应用范围
84

27

27

25

18

15

10

9

7

5

5

2

1

1

1
Western Blotting (WB), Proximity Ligation Assay (PLA)

原理

Rabbit polyclonal antibody raised against a full-length human RELB protein.

序列

MLRSGPASGP SVPTGRAMPS RRVARPPAAP ELGALGSPDL SSLSLAVSRS TDELEIIDEY IKENGFGLDG GQPGPGEGLP RLVSRGAASL STVTLGPVAP PATPPPWGCP LGRLVSPAPG PGPQPHLVIT EQPKQRGMRF RYECEGRSAG SILGESSTEA SKTLPAIELR DCGGLREVEV TACLVWKDWP HRVHPHSLVG KDCTDGICRV RLRPHVSPRH SFNNLGIQCV RKKEIEAAIE RKIQLGIDPY NAGSLKNHQE VDMNVVRICF QASYRDQQGQ MRRMDPVLSE PVYDKKSTNT SELRICRINK ESGPCTGGEE LYLLCDKVQK EDISVVFSRA SWEGRADFSQ ADVHRQIAIV FKTPPYEDLE IVEPVTVNVF LQRLTDGVCS EPLPFTYLPR DHDSYGVDKK RKRGMPDVLG ELNSSDPHGI ESKRRKKKPA ILDHFLPNHG SGPFLPPSAL LPDPDFFSGT VSLPGLEPPG GPDLLDDGFA YDPTAPTLFT MLDLLPPAPP HASAVVCSGG AGAVVGETPG PEPLTLDSYQ APGPGDGGTA SLVGSNMFPN HYREAAFGGG LLSPGPEAT

交叉反应

人

产品特性

Antibody reactive against mammalian transfected lysate.

免疫原

RELB (NP_006500.2, 1 a.a. ~ 579 a.a) full-length human protein.
anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 1-579) antibody
RELB 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (C-Term) antibody
RELB 适用: 人, 小鼠 WB, IP, IHC (p), ChIP 宿主: 兔 Polyclonal unconjugated

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (pSer573) antibody
RELB 适用: 人, 小鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 248-492) antibody
RELB 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 161-260) antibody
RELB 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) antibody
RELB 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal 4C5 unconjugated

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 400-579) antibody
RELB 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (pSer573) antibody
RELB 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (AA 1-579) antibody
RELB 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB) (N-Term) antibody
RELB 适用: 人, 犬, 豚鼠, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
生效 #104076 (Immunofluorescence)

by

Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie des Universitätsklinikums Knappschaftskrankenhaus Bochum

No.

#104076

日期

2019.11.21

抗原

RELB

Lot Number

09098

Method validated

Immunofluorescence

Positive Control

U-937 human monocytic cell line

Negative Control

No primary antibody control

Notes

Passed. ABIN519730 specifically stains RelB in U-937 human monocytic cells.

Validation Images

U937 monocytic cells stained with with anti-RelB antibody ABIN519730 and a TRITC-labeled secondary antibody (bottom row, red). Counterstain with DAPI (blue). Negative control without primary antibody (top row) shows no specific TRITC signal.
Full Methods
Primary Antibody

ABIN519730

Secondary Antibody

TRITC-conjugated AffiniPure donkey anti-rabbit IgG antibody (611-025-215, Jackson ImmunoResearch Europe Ltd.)

Full Protocol

Grow U-937 cells in RPMI 1640 Medium (Gibco, 21875034) supplemented with 10 % FCS (Gibco, A3840001) and Pen/Strep (Gibco, 15140122), at 37°C and 5% CO₂ in 300µL in 24-well plates (Sarstedt AG&Co. KG).
Use cytospin to spin cells onto slide.
Fix cells on coverslide in 4% paraformaldehyde (Carl Roth GmbH, 0335.1) for 30min at RT.
Wash cells 3x for 5min with PBS (Sigma-Aldrich, 8537).
Permeabilize cells in 100µL PBS containing 0.1 % Triton X-100 (Sigma-Aldrich, T8787) for 5min at RT.
Wash cells 3x for 5min with PBS.
Block non-specific binding with 1x Duolink Blocking solution (Sigma-Aldrich, DUO82007) for 30min at RT.
Incubate cells with primary rabbit anti-human RelB antibody (antibodies-online, ABIN519730, lot 09098 WUlz) diluted 1:100 in 1x Duolink Antibody diluent (Sigma-Aldrich, DUO82008) ON at 4°C.
Wash cells 3x for 5min with PBS.
Incubate cells with secondary TRITC-conjugated AffiniPure donkey anti-rabbit IgG antibody (611-025-215, Jackson ImmunoResearch Europe Ltd.) diluted 1:400 in 1x Duolink Antibody diluent for 1 h at RT in the dark.
Wash cells 2x for 5min with PBS.
Mount coverslips on glass slides in 50µL ProLong Gold antifade mountant with DAPI (Invitrogen, P36931).
Image acquisition with an Olympus widefield microscope.

Experimental Notes

Strong red staining depending on the presence of primary antibody indicates that the antibody works. Negative control lacking the primary antibody shows only background fluorescence.
缓冲液

In 1x PBS, pH 7.4

注意事项

Aliquot to avoid repeated freezing and thawing.

储存条件

-20 °C

储存方法

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
抗原

RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))

别名

RELB (RELB 产品)

别名

shep antibody, I-REL antibody, IREL antibody, REL-B antibody, XRelB antibody, relb-A antibody, GC-rich antibody, avian reticuloendotheliosis viral (v-rel) oncogene related B antibody, RELB proto-oncogene, NF-kB subunit antibody, v-rel avian reticuloendotheliosis viral oncogene homolog B S homeolog antibody, Relb antibody, RELB antibody, relb.S antibody

背景

Full Gene Name: v-rel reticuloendotheliosis viral oncogene homolog B
Synonyms: I-REL,IREL

基因ID

5971

NCBI登录号

NM_006509

途径

NF-kappaB Signaling, RTK signaling