Sulfotyrosine 抗体 (Atto 488)
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- 抗原 See all Sulfotyrosine products
- Sulfotyrosine
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Sulfotyrosine antibody is conjugated to Atto 488
- 应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
- 特异性
- Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
- 纯化方法
- Protein G Purified
- 免疫原
- Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
- 克隆位点
- 7C5
- 亚型
- IgG1
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- IHC (1:25)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN5067489 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- Sulfotyrosine
- Abstract
- Sulfotyrosine 产品
- 物质类
- Amino Acid
- 背景
- Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.
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