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Sulfotyrosine 抗体

WB, ELISA, ICC, IF, IHC 宿主: 小鼠 Monoclonal 7C5 unconjugated
产品编号 ABIN5067487
发货至: 中国
  • 抗原 See all Sulfotyrosine products
    Sulfotyrosine
    宿主
    • 10
    小鼠
    克隆类型
    • 10
    单克隆
    标记
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Sulfotyrosine antibody is un-conjugated
    应用范围
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    特异性
    Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
    纯化方法
    Protein G Purified
    免疫原
    Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
    克隆位点
    7C5
    亚型
    IgG1
  • 应用备注
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • IHC (1:25)
    • optimal dilutions for assays should be determined by the user.
    说明

    A 1:1000 dilution of ABIN5067487 was sufficient for detection of Sulfotyrosine in 0.5 μg of Sulfotyrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    -20°C
  • 抗原
    Sulfotyrosine
    Abstract
    Sulfotyrosine 产品
    物质类
    Amino Acid
    背景
    Tyrosine sulfation is a common post-translational protein modification catalyzed by tyrosylprotein sulfotransferase (TPST) (1). Sulfotyrosines mediate interactions between hirudin and thrombin, P-selectin glycoprotein ligand-1 and P-selectin, and von Willebrand factor and factor VIII. Tyrosine sulfation of certain receptors is required for several viruses and parasites to enter their hosts.
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