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HNE 抗体 (Atto 594)

HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 Atto 594
产品编号 ABIN5067293
发货至: 中国
  • 抗原 See all HNE 抗体
    HNE (4-Hydroxynonenal (HNE))
    宿主
    • 15
    • 11
    • 1
    小鼠
    克隆类型
    • 16
    • 11
    单克隆
    标记
    • 5
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    • 1
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    • 1
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    This HNE antibody is conjugated to Atto 594
    应用范围
    • 26
    • 13
    • 13
    • 9
    • 9
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
    特异性
    Specific for 4-HNE modified proteins. Does not detect free 4-Hydroxynonenal. Does not X-react with 4-HHE, Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
    纯化方法
    Protein G Purified
    免疫原
    Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
    克隆位点
    12F7
    亚型
    IgG1
    Top Product
    Discover our top product HNE Primary Antibody
  • 应用备注
    • WB (1:1000)
    • ICC/IF (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    说明

    A 1:1000 dilution of ABIN5067293 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Conjugated antibodies should be stored at 4°C
  • 抗原
    HNE (4-Hydroxynonenal (HNE))
    别名
    4-Hydroxynonenal (HNE 产品)
    物质类
    Chemical
    背景
    4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
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