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- 抗原 See all HNE 抗体
- HNE (4-Hydroxynonenal (HNE))
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HNE antibody is conjugated to Atto 390
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应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Specific for 4-HNE modified proteins. Does not detect free 4-Hydroxynonenal. Does not X-react with 4-HHE, Acrolein, Crotonaldehyde, Hexanoyl Lysine, MDA, or Methylglyoxal modified proteins.
- 纯化方法
- Protein G Purified
- 免疫原
- Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
- 克隆位点
- 12F7
- 亚型
- IgG1
- Top Product
- Discover our top product HNE Primary Antibody
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anti-4-Hydroxynonenal (HNE) antibody
HNE WB, ELISA, IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-4-Hydroxynonenal (HNE) antibodyHNE WB, ELISA, IHC 宿主: 小鼠 Monoclonal HNEJ-2 unconjugated
anti-4-Hydroxynonenal (HNE) antibodyHNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 unconjugated
anti-4-Hydroxynonenal (HNE) antibody (HRP)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 HRP
anti-4-Hydroxynonenal (HNE) antibody (FITC)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 FITC
anti-4-Hydroxynonenal (HNE) antibody (Biotin)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 Biotin
anti-4-Hydroxynonenal (HNE) antibody (PE)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 PE
anti-4-Hydroxynonenal (HNE) antibody (PerCP)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 PerCP
anti-4-Hydroxynonenal (HNE) antibody (APC)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 APC
anti-4-Hydroxynonenal (HNE) antibody (Atto 594)HNE WB, ELISA, ICC, IF 宿主: 小鼠 Monoclonal 12F7 Atto 594
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN5067290 was sufficient for detection of 4-Hydroxynonenal in 0.5 μg of 4-Hydroxynonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- HNE (4-Hydroxynonenal (HNE))
- 别名
- 4-Hydroxynonenal (HNE 产品)
- 物质类
- Chemical
- 背景
- 4-Hydroxy-2-nonenol (4-HNE) is an unsaturated aldehyde derived from lipid peroxidation. 4-HNE is an electrophile and reacts with protein nucleophiles such as cysteine, histine, and lysine (1). Low levels of 4-HNE promote cell survival via cellular antioxidant induction whereas higher levels lead to autophagy, apoptosis, and ultimately necrosis. 4-HNE has been linked to Alzheimer's disease, Parkinson's disease, cancer, cardiovascular diseases, diabetes, and liver disease.
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