4-Hydroxy-2-Hexenal (4-HHE) 抗体 (Atto 390)
Quick Overview for 4-Hydroxy-2-Hexenal (4-HHE) 抗体 (Atto 390) (ABIN5067272)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for 4-HHE and 4-HNE modified proteins. Does not detect free 4-HHE or 4-HNE. Does not X-react with Acrolein, Crotonaldehyde, HEL, MDA, or Methylglyoxal modified proteins.
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纯化方法
- Protein G Purified
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免疫原
- Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG2b
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anti-4-Hydroxy-2-Hexenal (4-HHE) antibody
ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 6F10 unconjugated
anti-4-Hydroxy-2-Hexenal (4-HHE) antibody (HRP)ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 6F10 HRP
anti-4-Hydroxy-2-Hexenal (4-HHE) antibody (FITC)ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 6F10 FITC
anti-4-Hydroxy-2-Hexenal (4-HHE) antibody (Biotin)ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 6F10 Biotin
anti-4-Hydroxy-2-Hexenal (4-HHE) antibody (PE)ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 6F10 PE
anti-4-Hydroxy-2-Hexenal (4-HHE) antibody (PerCP)ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 6F10 PerCP
anti-4-Hydroxy-2-Hexenal (4-HHE) antibody (APC)ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 6F10 APC
anti-4-Hydroxy-2-Hexenal (4-HHE) antibody (Atto 488)ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 6F10 Atto 488
anti-4-Hydroxy-2-Hexenal (4-HHE) antibody (Atto 594)ELISA, WB, FACS, ICC, IF 宿主: 小鼠 Monoclonal 6F10 Atto 594
anti-4-Hydroxy-2-Hexenal (4-HHE) antibodyELISA, WB, IHC, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- FACS (1:50)
- FCM (1:50)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN5067272 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 μg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- 4-Hydroxy-2-Hexenal (4-HHE)
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别名
- 4-Hydroxy-2-hexenal
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物质类
- Chemical
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背景
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.
抗原
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