Acrolein 抗体 (HRP)
Quick Overview for Acrolein 抗体 (HRP) (ABIN5067194)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Specific for Acrolein modified proteins. Does not detect free acrolein. Does not X-react with Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, MDA, or Methylglyoxal modified proteins.
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纯化方法
- Protein G Purified
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免疫原
- Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG1
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anti-Acrolein (ACR) antibody
ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 unconjugated
anti-Acrolein (ACR) antibody (Biotin)ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 Biotin
anti-Acrolein (ACR) antibody (FITC)ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 FITC
anti-Acrolein (ACR) antibody (PE)ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 PE
anti-Acrolein (ACR) antibody (PerCP)ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 PerCP
anti-Acrolein (ACR) antibody (APC)ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 APC
anti-Acrolein (ACR) antibody (Atto 594)ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 Atto 594
anti-Acrolein (ACR) antibody (Atto 488)ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 Atto 488
anti-Acrolein (ACR) antibody (Atto 390)ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 Atto 390
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应用备注
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- WB (1:1000)
- ICC/IF (1:50)
- FACS (1:50)
- FCM (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN5067194 was sufficient for detection of Acrolein in 2 μg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- Acrolein (ACR)
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别名
- Acrolein
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物质类
- Chemical
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背景
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Acrolein is an electrophilic conjugated aldehyde that is a terminal product of lipid peroxidation. Acrolein is highly mutagenic and reacts with nucleophilic functional groups in DNA and proteins such as cysteine, histidine, and lysine residues (1).
抗原
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