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Acrolein 抗体 (Atto 488)

ACR ELISA, FACS, WB, ICC, IF, IHC 宿主: 小鼠 Monoclonal 10A10 Atto 488
产品编号 ABIN5067183
发货至: 中国
  • 抗原 See all Acrolein (ACR) products
    Acrolein (ACR)
    宿主
    • 9
    小鼠
    克隆类型
    • 9
    单克隆
    标记
    • 1
    • 1
    • 1
    • 1
    • 1
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    This Acrolein antibody is conjugated to Atto 488
    应用范围
    ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
    特异性
    Specific for Acrolein modified proteins. Does not detect free acrolein. Does not X-react with Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, MDA, or Methylglyoxal modified proteins.
    纯化方法
    Protein G Purified
    免疫原
    Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH).
    克隆位点
    10A10
    亚型
    IgG1
  • 应用备注
    • WB (1:1000)
    • ICC/IF (1:50)
    • FACS (1:50)
    • FCM (1:50)
    • ELISA (1:1000)
    • optimal dilutions for assays should be determined by the user.
    说明

    A 1:1000 dilution of ABIN5067183 was sufficient for detection of Acrolein in 2 μg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Conjugated antibodies should be stored at 4°C
  • 抗原
    Acrolein (ACR)
    别名
    Acrolein (ACR 产品)
    物质类
    Chemical
    背景
    Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Acrolein is an electrophilic conjugated aldehyde that is a terminal product of lipid peroxidation. Acrolein is highly mutagenic and reacts with nucleophilic functional groups in DNA and proteins such as cysteine, histidine, and lysine residues (1).
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