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MFN1 抗体 (AA 1-234)

Name: MFN1 抗体 (AA 1-234)
SKU: ABIN5013951
Availability: OutOfStock
Rating: 5 (1 reviews)

MFN1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Mitofusin 1 (MFN1) (AA 1-234) antibody (ABIN5013951) (MFN1 抗体 (AA 1-234))

IHC

5 images

(1 validation)

产品编号 ABIN5013951

发货至: 中国

Datasheet as PDF

抗原 See all MFN1 抗体

MFN1 (Mitofusin 1 (MFN1))
抗原表位
16

7

7

7

6

4

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 1-234
适用
51

30

27

21

16

5

4

4

3

2

2

1

1

1

1
小鼠
宿主
61

5

3

1
兔
Compatible Secondaries
克隆类型
58

12
多克隆
标记
33

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This MFN1 antibody is un-conjugated
应用范围
53

26

15

11

6

5

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

原理

Polyclonal Antibody to Mitofusin 1 (MFN1)

特异性

The antibody is a rabbit polyclonal antibody raised against MFN1. It has been selected for its ability to recognize MFN1 in immunohistochemical staining and western blotting.

交叉反应

人, 大鼠

纯化方法

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

免疫原

Recombinant Mitofusin 1 (MFN1) corresdonding to Met1~Ile234

亚型

IgG

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MFN1 适用: 人, 兔, 犬, 马, 猴 IHC, IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Mitofusin 1 (MFN1) (AA 622-741) antibody
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MFN1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

应用备注

Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.

说明

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

限制

仅限研究用
生效 #103013 (Western Blotting)

by

Wang Lab, Department of Animal Science, College of Agriculture & Natural Resources

No.

#103013

日期

2018.05.30

抗原

MFN1

Lot Number

A20180411903

Method validated

Western Blotting

Positive Control

293T cells transfected with MFN1-HA expression vector; the full length MFN1 CDS has been inserted upstream of an HA-tag in plasmid pcDNA3.1.

Negative Control

293T cells transfected with an LUC-HA expression vector, and the full length LUC CDS has been inserted upstream of an HA-tag in plasmid pcDNA3.1.

293T cells transfected with MFN2-HA expression vector; the full length MFN2 CDS has been inserted upstream of an FLAG-tag in plasmid pcDNA3.1.

Notes

Passed. ABIN5013951 specifically recognizes antigen in sample.

Validation Images

Western blot analysis of lysates from 293T cells ectopically expressing HA-tag labeled human MFN1 (MFN1-HA), luciferase (LUC-HA), and human MFN2 (MFN2-HA). Protein bands were revealed using ABIN5013951, an HA-tag antibody, an MFN2 antibody, and a beta-actin loading control.
Full Methods
Primary Antibody

ABIN5013951

Secondary Antibody

goat anti-rabbit IgG (H+L) antibody HRP conjugate (Bio-Rad, 170-6515)

Full Protocol

Grow 293T cells in DMEM medium (Gibco, lot 1897400) supplemented with 10% fetal bovine serum (Gibco, lot 1913756) and 1% Penicillin-Streptomycin (thermo, 10378016), at 37°C and 7% CO₂ in 6-well plates to 60-70% confluency.
Transfect cells with plasmids expressing either MFN1-HA, MFN2-HA or LUC-HA with max 2µg of plasmid using PEI transfection reagent (Poly-Science, 24765-1) following the manufacturer´s instructions.
Lyse cells in 100µl RIPA buffer containing protease inhibitors (Sigma-Aldrich, P8340).
Determine total protein content of the lysates using a Bradford protein assay (Protein Assay Dye Reagent Concentrate, Bio-Rad, 5000006).
Denature 100µg total protein for 10min at 95°C in 1x SDS loading buffer and subsequently separate them on a freshly cast 10% denaturing polyacrylamide gel.
Transfer proteins onto Immun-Blot Low Fluorescence PVDF Membrane (0.45μm) at 1.0A and up to 25V for 30min via Trans-Blot Turbo Transfer System.
Block the membrane with Bio-rad Blotting-Grade Blocker (5% non-fat milk in PBS).
Incubation with primary
rabbit anti-MFN1 antibody (antibodies-online, ABIN5013951, lot A20180411903) diluted 1:200 in Blocking Buffer (5% non-fat milk) ON at 4°C.
mouse anti-HA-tag antibody (Santa Cruz, sc-7392) diluted 1:2000 in Blocking Buffer (5% non-fat milk) ON at 4°C (only LUC-HA expressing cells).
mouse anti-MFN2-tag antibody (Santa Cruz, sc-100560) diluted 1:2000 in Blocking Buffer (5% non-fat milk) ON at 4°C.
mouse anti-beta-actin antibody (Sigma-Aldrich, A1978) diluted 1:10000 in Blocking Buffer(5% non-fat milk) ON at 4°C.
Wash membrane 3x for 10min with PBS supplemented with 0.1% Tween 20.
Incubation with secondary goat anti-rabbit IgG (H+L) antibody HRP conjugate (Bio-Rad, 170-6515) or goat anti-mouse IgG (H+L) antibody HRP conjugate (Bio-Rad, 170-6516) diluted 1:10000 in Odyssey Blocking Buffer for 1h at RT.
Wash membrane 3x for 10min with PBS supplemented with 0.1% Tween 20.
Add Clarity and Clarity Max Western ECL Blotting Substrates and scan the membrane on a Bio-Rad ChemiDoc XRS+System.

Experimental Notes

The antigen antibody ABIN5013951 reveals a protein of the expected molecular weight of antigen in lysates of cells. The protein bands is only visible in the positive but not the negative controls.
状态

Liquid

浓度

Lot specific

缓冲液

0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

储存液

ProClin

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

有效期

24 months
抗原

MFN1 (Mitofusin 1 (MFN1))

别名

Mitofusin 1 (MFN1 产品)

别名

mfn1 antibody, zgc:66150 antibody, mitofusin-1 antibody, MFN1 antibody, MFN2 antibody, hfzo1 antibody, hfzo2 antibody, 2310002F04Rik antibody, 6330416C07Rik antibody, D3Ertd265e antibody, HR2 antibody, mKIAA4032 antibody, Fzo1b antibody, mitofusin 1b antibody, mitofusin 1 antibody, mitofusin 1 S homeolog antibody, mfn1b antibody, MFN1 antibody, mfn1.S antibody, mfn1 antibody, Mfn1 antibody

背景

Hfzo1, Hfzo2, Transmembrane GTPase MFN1, Fzo homolog