ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
抗原
PIGY
(Phosphatidylinositol Glycan Anchor Biosynthesis, Class Y (PIGY))
PIG-Y antibody, pig-y antibody, Prey antibody, Pyurf antibody, prey antibody, 1810008A14Rik antibody, Pigy antibody, phosphatidylinositol glycan anchor biosynthesis class Y antibody, phosphatidylinositol glycan anchor biosynthesis, class Y antibody, Pigy upstream reading frame S homeolog antibody, phosphatidylinositol glycan anchor biosynthesis, class Y-like antibody, PIGY antibody, pigy antibody, Pigy antibody, pyurf.S antibody, Pigyl antibody
背景
Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.Synonyms: PIG-Y, Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y, Phosphatidylinositol-glycan biosynthesis class Y protein