Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
特异性
This antibody detects PERP.
交叉反应 (详细)
Species reactivity (tested):Human
纯化方法
DEAE purified
免疫原
PERP antibody was raised against a synthetic peptide corresponding to amino acids near the carboxy terminus of human PERP, which differ from the mouse sequence by three amino acids (7-9).
ELISA. Western blot. Immunoflourescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6), MTBP (7) and PERP (8), were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein (8-10). p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis (8). The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family (8).Synonyms: KCP-1, KCP1, KRTCAP1, Keratinocyte-associated protein 1, P53-induced protein PIGPC1, PERP, PIGPC1, THW, Transmembrane protein THW, p53 apoptosis effector related to PMP-22