Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
特异性
IRAK2 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy-terminus of mouse IRAK2a which is common to all four isoforms. mIRAK2 antibody will also recognize human IRAK2. Anti-IRAK2 has no cross response to IRAK. Anti-IRAK2 will also recognize human IRAK2.
纯化方法
Affinity chromatography purified via peptide column
ELISA. Western Blot: 0.5 - 1 μg/mL. A 65 kDa band should be detected. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappaB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family has been identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kappaB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-?B activation (2,3). Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion (4). IRAK2 is expressed in a variety of tissues.Synonyms: IRAK-2, Interleukin-1 receptor-associated kinase-like 2