Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
特异性
IL-1RAcP antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of IL-1RAcP, which differs from the sequence of mouse origin by three amino acids. It has no cross activity to other member in the IL-1 receptor family.
纯化方法
Affinity chromatography purified via peptide column
ELISA. Western Blot: 1/500 to 1/1000. An approximately 66 kDa band can be detected. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I(IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1).IL-1 RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kappaB inducing kinae (NIK) and of NF-kappaB, recruites to the IL-1R complex through IL-1 RAcP(2). IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP(3,4). Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.Synonyms: EMI2, Endogenous meiotic inhibitor 2, F-box only protein 43, FBP5B