Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
特异性
IKK alpha antibody was raised against a peptide corresponding to amino acids 699 to 715 of human IKK alpha, which differs from corresponding murine sequence by one amino acid. This polyclonal antibody has no cross response to IKKb or IKKy.
纯化方法
Affinity chromatography purified via peptide column
CHUK
适用: 小鼠, 大鼠
ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA. Western Blot: 1: 500 to 1: 1000 dilution. A 85 kDa band should be detected. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and bacteria product LPS. NF-kappaB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The long-sought IkappaB kinase (IKK), which phosphorylates IkappaB, and mediates IkappaB degradation and NF-kappaB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKΒ). IKKalpha and IKKb interact with each other and both are essential for the NF-kappaB activation. IKKalpha specifically phosphorylates IkappaB-?.IKKalpha is expressed in a variety of human tissues.Synonyms: CHUK, Conserved helix-loop-helix ubiquitous kinase, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK-alpha, IKK1, IkBKA, IkappaB kinase, Inhibitor of nuclear factor kappa-B kinase subunit alpha, NFKBIKA, Nuclear factor NF-kappa-B inhibitor kinase alpha, TCF16