DEDAF antibody was raised against a synthetic peptide corresponding to amino acids 215 to 228 of human DEDAF. The sequence is identical to that of mouse origin.
纯化方法
Affinity chromatography purified via peptide column
ELISA. Western Blot: 0.5 to 1 μg/mL. A band at approximately 32 kDa can be detected. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
AAP1 antibody, DEDAF antibody, YEAF1 antibody, 2410018J24Rik antibody, rybp antibody, aap1 antibody, dedaf antibody, yeaf1 antibody, cb337 antibody, wu:fi34f03 antibody, Dedaf antibody, zgc:153842 antibody, RING1 and YY1 binding protein antibody, RING1 and YY1 binding protein L homeolog antibody, RING1 and YY1 binding protein a antibody, RING1 and YY1 binding protein b antibody, RYBP antibody, Rybp antibody, rybp antibody, rybp.L antibody, rybpa antibody, rybpb antibody
背景
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor . DEDAF is identical to the transcriptional repressor RYBP (2). DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.Synonyms: AAP-1, APAP-1, Apoptin-associating protein 1, DED-associated factor, DEDAF, Death effector domain-associated factor, RING1 and YY1-binding protein, YEAF1, YY1 and E4TF1-associated factor 1