5-Methylcytosine 抗体 (AbBy Fluor® 750)
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- 抗原
- 5-Methylcytosine
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- AbBy Fluor® 750
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- 交叉反应
- 人
- 交叉反应 (详细)
- 5 MethylCytosine
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated 5 MethylCytosine
- 亚型
- IgG
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- 应用备注
- IF(IHC-P) 1:50-200
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- 5-Methylcytosine
- 别名
- 5 MethylCytosine
- 物质类
- Chemical
- 背景
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Synonyms: 5-Methyl Cytidine, 5 m C, 5 mC, 5 me C, 5 Me Cytidine, 5 MeCyd, 5 Methyl Cytidine, 5 MethylCytosine, 5-mC, 5-Me Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine.
Background: Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation.
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