ELISA. Western Blot: Casp-5 antibody can be used for the detection of caspase-5 at 0.5 - 1 μg/mLDepending on cell lines or tissues used, other cleavage products may be observed. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18 (2,3). Caspase-5 can interact with caspase-1, both are constituents of the NALP1 inflammasome, a complex that can trigger the cleavage of pro-IL-1beta (4). Expression of caspase-5 can be regulated by lipopolysaccharide (LPS) and IFN-gamma (5).Synonyms: CASP-5, CASP5, ICE(rel)-III, ICH-3 protease, ICH3, TY protease