Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
特异性
CARD9 antibody was raised against a synthetic peptide corresponding to amino acids 521 to 536 of human CARD9 The sequence is different from that of rat origin by two amino acids.
纯化方法
Affinity chromatography purified via peptide column
ELISA. Western Blot: CARD9 antibody can be used for detection of CARD9 at 0.5 to 1 μg/mL. A bandat approximately 59 kDa can be detected. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C.
抗原
CARD9
(Caspase Recruitment Domain Family, Member 9 (CARD9))
Gm782 antibody, card9 antibody, CANDF2 antibody, hCARD9 antibody, caspase recruitment domain family, member 9 antibody, caspase recruitment domain family member 9 antibody, Card9 antibody, CARD9 antibody, card9 antibody
背景
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD9, which interacts with the CARD activation domain of BCL-10 (1). CARD9 associates with BCL-10 and forms a complex within cells. CARD9 induces apoptosis and activates NF-kappaB. CARD9 is an upstream activator of BCL-10 and NF-kappaB signaling.Synonyms: Caspase recruitment domain-containing protein 9