ELISA. Western Blot: BANF1 antibody can be used for detection of BANF1 at 0.5 - 1 μg/mL. Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C.
抗原
BANF1
(Barrier To Autointegration Factor 1 (BANF1))
Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BAF also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BAF will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BAF is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA. Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE.Synonyms: BAF, BCRG1, Barrier-to-autointegration factor, Breakpoint Cluster Region Protein 1