ELISA. Western Blot: Bad antibody can be used for detection of Bad at 0.5 to 1 μg/mL. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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背景
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death (reviewed in 1 and 2). Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax (3). Bad activity is regulated by its phosphorylation, it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival (4), whereas JNK-induced phosphorylation promotes the apoptotic role of Bad (5).Synonyms: BAD, BBC6, BCL2L8, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8