ELISA. Western Blot: ARC antibody can be used for detection of ARC at 1/1000 dilution. Anapproximately 25 kDa band can be detected. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C.
抗原
NOL3
(Nucleolar Protein 3 (Apoptosis Repressor with CARD Domain) (NOL3))
Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD (1). ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue (1).Synonyms: Apoptosis repressor with CARD, Muscle-enriched cytoplasmic protein, Myp, NOP, Nop30, Nucleolar protein 3, Nucleolar protein of 30 kDa