ELISA. Western Blot: AP2M1 antibody can be used for detection of AP2M1 at 1 - 2 μg/mL. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % sodium azide.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store the antibody undiluted at 2-8 °C.
抗原
AP2M1
(Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))
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背景
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.Synonyms: AP-2 mu-2 chain, Adaptin-mu2, CLAPM1, Clathrin assembly protein complex 2 medium chain, Clathrin coat assembly protein AP50, Clathrin coat-associated protein AP5, HA2 50 kDa subunit, KIAA0109, Plasma membrane adaptor AP-2 50 kDa protein