Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
特异性
Acinus antibody was raised against a peptide corresponding to amino acids 775 to 789 of Human AcinusL, 48 to 62 of human AcinusS', or 17 to 31 of human AcinusS, which differ from those of mouse Acinus by one amino acid .
纯化方法
Affinity chromatography purified via peptide column
ELISA. Western Blot: Acinus antibody can be used for detection of Acinus at 0.5 to 1 μg/mL. A 220 kDa band can be detected. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
缓冲液
PBS containing 0.02 % Sodium Azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE . A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS (2).Synonyms: Apoptotic chromatin condensation inducer in the nucleus, KIAA0670