Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, incuding LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and Cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morpho-genesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and it is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.Synonyms: EC=2.7.11.1, LIM domain kinase 2, LIMK-2, LIMK2