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Vimentin 抗体

Name: Vimentin 抗体
SKU: ABIN498219
Availability: OutOfStock

VIM 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

Western blot analyzes of Vimentin antibody in extracts from A549 cells. (Vimentin 抗体)

Immunohistochemistry analyzes of Vimentin antibody in paraffin-embedded human breast carcinoma tissue. (Vimentin 抗体)

IHC (p)

2 images

产品编号 ABIN498219

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
适用
305

120

117

60

60

49

42

37

29

21

20

13

13

8

8

6

6

3

3

2

1

1

1

1

1

1

1

1

1
人
宿主
158

130

11

6

6

5

2
兔
Compatible Secondaries
克隆类型
172

145
多克隆
标记
198

15

13

12

9

7

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
263

115

104

97

88

64

48

40

39

33

23

20

8

5

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

特异性

This antibody detects endogenous levels of Vimentin protein.

交叉反应（详细）

Species reactivity (tested):Human.

纯化方法

Affinity chromatography using epitope-specific immunogen

纯度

> 95 % (by SDS-PAGE)

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anti-Vimentin (VIM) antibody
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anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 小鼠, 大鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C8 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

anti-Vimentin (VIM) (AA 210-466) antibody
VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原

Vimentin (VIM)

别名

Vimentin (VIM 产品)

背景

Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.Synonyms: VIM

分子量

approx. 57 kDa

基因ID

7431

NCBI登录号

NP_003371

UniProt

P08670

途径

Caspase Cascade in Apoptosis