Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
This antibody detects endogenous levels of RFA2 protein.
交叉反应 (详细)
Species reactivity (expected):Mouse. Species reactivity (tested):Human.
纯化方法
Affinity chromatography using epitope-specific immunogen
纯度
> 95 % (by SDS-PAGE)
免疫原
synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.
Western blot: 1: 500 - 1: 1000. immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This phospho specific polyclonal antibody is specific for phosphorylated pS122 of yeast RFA2. Reactivity with non-phosphorylated yeast RFA2 is minimal by ELISA. Immunogen: synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.Synonyms: REPA2, RF-A protein 2, RFA2, RP-A p32, RP-A p34, RPA32, RPA34, Replication factor A protein 2, Replication protein A, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit