Peptide ELISA: Limit Dilution: 1/4000. Western blot: 1-3 μg/mL. Approx 48 kDa band observed in Mouse and Rat Spinal Cordlysates (calculated MW of 50.8 kDa according to Human NP_006227.1 and 50.8 kDaaccording to Mouse NP_032926.2). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
BRN1 antibody, OTF8 antibody, Brn1 antibody, RHS1 antibody, Brn-1 antibody, Otf8 antibody, Skin1 antibody, brn1 antibody, nrl-20 antibody, nrl20-A antibody, pou1 antibody, pou23 antibody, wu:fb92g06 antibody, zfpou1 antibody, zp23 antibody, zp23pou antibody, BRN-1.1 antibody, ZP12POU antibody, brn1.1 antibody, pou12 antibody, wu:fc33a11 antibody, zp12 antibody, POU class 3 homeobox 3 antibody, POU domain, class 3, transcription factor 3 antibody, POU class 3 homeobox 3 S homeolog antibody, POU class 3 homeobox 3b antibody, POU class 3 homeobox 3a antibody, POU3F3 antibody, Pou3f3 antibody, pou3f3.S antibody, pou3f3b antibody, pou3f3a antibody
背景
POU3F3, also known as Brn1, is a member of the POU family of transcription factors that is predominantly expressed in the brain. It contains a characteristic POU domain and a homeobox DNA-binding domain. POU3F3 plays a role in the development of the brain and kidney by upregulating transcription of key genes in these tissues. For example, POU3F3 interacts with the proneural protein Mash1 and POU3F2 (Brn2) on the promoter of the Notch ligand Delta1 to activate Delta1 transcription, a key step in neurogenesis.Synonyms: BRN1, Brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, OTF-8, OTF8, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, POU domain class 3 transcription factor 3