Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
免疫原
This Lamin B2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 483-512 amino acids from the C-terminal region of Human Lamin B2. Genename: LMNB2
ELISA: 1/1,000Western blotting: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
0.25 mg/mL
缓冲液
PBS with 0.09 % (W/V) Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Lamin B2 is one of the two B type proteins, B2.Synonyms: LMN2, LMNB, LMNB-2, LMNB2, Lamin-B2, Nuclear Envelope Marker