Western Blotting (WB), ELISA, Immunofluorescence (IF)
纯化方法
Antigen affinity purified
免疫原
This TLE1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 187-221 amino acids from the N-terminal region of human TLE1.
Titration of the TLE1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:25,Western blot: 1:1000
限制
仅限研究用
状态
Liquid
缓冲液
In 1X PBS, pH 7.4, with 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot the TLE1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
抗原
TLE1
(Transducin-Like Enhancer of Split 1 (E(sp1) Homolog, Drosophila) (TLE1))
Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.