Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
交叉反应 (详细)
Expected species reactivity: Bovine
纯化方法
Antigen affinity purified
免疫原
This SMAD1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 163-196 amino acids from the Central region of human SMAD1.
Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:25,IHC (Paraffin): 1:25,Western blot: 1:1000
限制
仅限研究用
状态
Liquid
缓冲液
In 1X PBS, pH 7.4, with 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot the SMAD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
抗原
SMAD1
(SMAD, Mothers Against DPP Homolog 1 (SMAD1))
SMADs are intracellular proteins that transduce extracellular signals from transforming growth factor beta ligands to the nucleus where they activate downstream gene transcription. The SMADs, which form a trimer of two receptor-regulated SMADs and one co-SMAD, act as transcription factors that regulate the expression of certain genes. There are three classes of SMADs, SMAD1 belongs to the receptor-regulated class (R-SMAD) which includes SMAD2, SMAD3, SMAD5 and SMAD8/9. [Wiki]