Western Blotting (WB), ELISA, Immunofluorescence (IF), Dot Blot (DB)
纯化方法
Antigen affinity purified
免疫原
This phospho-ERBB2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1005 of human ERBB2.
Titration of the phospho-ERBB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:100,Dot blot: 1:500
限制
仅限研究用
状态
Liquid
缓冲液
In 1X PBS, pH 7.4, with 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot the phospho-ERBB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.