Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
交叉反应 (详细)
Expected species reactivity: Mouse, Rat, Bovine
纯化方法
Antigen affinity purified
免疫原
This phospho-CDC25A antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT507 of human CDC25A.
Titration of the phospho-CDC25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:25,Western blot: 1:250-1000,IHC (Paraffin): 1:50-1:100
限制
仅限研究用
状态
Liquid
缓冲液
In 1X PBS, pH 7.4, with 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot the phospho-CDC25A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
抗原
CDC25A
(Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.