Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
纯化方法
Antigen affinity purified
免疫原
This p-p21 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT145 of human P21CIP1.
CDKN1A
适用: 人
ELISA, IHC, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
Titration of the p-p21 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Dot blot: 1:500,IHC (Paraffin): 1:50-1:100
限制
仅限研究用
状态
Liquid
缓冲液
In 1X PBS pH 7.4 with 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot the p-p21 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
P21CIP1 is a potent cyclin-dependent kinase inhibitor. This protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21CIP1 can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein has been reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.