Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
纯化方法
Antigen affinity purified
免疫原
This GLI2 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 1287-1321 amino acids from the C-terminal region of human GLI2.
Titration of the GLI2 antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. GLI-2 exists as five alternatively spliced isoforms of 133 kDa (isoform 1), 132 (2), 88 (3), 86 (4) and 168 kDa (isoform 5).\. Immunofluorescence: 1:25,Flow Cytometry: 1:25,Western blot: 1:1000
限制
仅限研究用
状态
Liquid
缓冲液
In 1X PBS, pH 7.4, with 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot the GLI2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Acts as a transcriptional activator. May play a role during embryogenesis. Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. Is involved in the smoothened (SHH) signaling pathway.