Topoisomerase II alpha 抗体 (AA 1352-1493)
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- 抗原 See all Topoisomerase II alpha (TOP2A) 抗体
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
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抗原表位
- AA 1352-1493
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Topoisomerase II alpha antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.
- 克隆位点
- TOP2A-1361
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product TOP2A Primary Antibody
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- 应用备注
- The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Topoisomerase II alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))
- 别名
- TOP2A / Topoisomerase II alpha (TOP2A 产品)
- 背景
- This mAb recognizes an ~170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
- 基因ID
- 7153
- 途径
- Cell Division Cycle, Mitotic G1-G1/S Phases
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