The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,IF: 1-2 μg/mL,IHC (FFPE): 0.1-0.2 μg/mL for 30 min at RT
限制
仅限研究用
浓度
1 mg/mL
缓冲液
1 mg/mL in 1X PBS, BSA free, sodium azide free
储存液
Azide free
储存条件
4 °C,-20 °C
储存方法
Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Recognizes a protein of about 50 kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.