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Syntaxin 6 抗体

STX6 适用: 小鼠, 大鼠, Hamster WB, IF, IP 宿主: 小鼠 Monoclonal 3D10 unconjugated
产品编号 ABIN492572
发货至: 中国
  • 抗原 See all Syntaxin 6 (STX6) 抗体
    Syntaxin 6 (STX6)
    适用
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    小鼠, 大鼠, Hamster
    宿主
    • 63
    • 5
    • 2
    • 2
    小鼠
    克隆类型
    • 67
    • 5
    单克隆
    标记
    • 26
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    This Syntaxin 6 antibody is un-conjugated
    应用范围
    • 51
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    Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
    特异性
    This antibody reacts with 31 kDa membrane protein Syntaxin-6 on Western blotting and Immunoprecipitation. It is reported that this monoclonal antibody (3D10) binds to the amino-terminal 25 amino acid of Rat Syntaxin-6 (Ref.6). Detects a band of approximately 35 kDa (predicted molecular weight: 30.6 kDa).
    交叉反应 (详细)
    Species reactivity (tested):Mouse, Hamster and Rat.It is reported that this clone 3D10 reacted with Human Syntaxin-6 in Reference 3.
    产品特性
    Synonyms: Syntaxin-6, Golgi Marker
    纯化方法
    Protein-A Sepharose Chromatography of hybridoma supernatant.
    免疫原
    Recombinant Rat Syntaxin-6. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/cmouse splenocyte
    克隆位点
    3D10
    亚型
    IgG1
    Top Product
    Discover our top product STX6 Primary Antibody
  • 应用备注
    Western blotting: 1 μg/mL for chemiluminescence detection system. Immunoprecipitation: 2 μg/300 μL of cell extract. Immunocytochemistry. Detailed procedure is provided in Protocols.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    实验流程
    SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10%glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the cold Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 3 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (Theconcentration of antibody will depend on condition. )8) Wash the membrane with PBS-T [0. 05% Tween-20 in PBS] (5 minutes x 3 times). 9) Incubate the membrane with the 1: 10,000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 10) Wash the membrane with PBS-T (10 minutes x 3 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriatechemiluminescence reagent for 1 minute. 12) Remove extra reagent from the membrane by dabbing with paper towel, and seal it inplastic wrap. 13) Expose to an X-ray film in a dark room for 3 minutes. 14) Develop the film as usual. The condition for exposure and development may vary. Positive Controls: Rat brain, WR19LImmunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, pH 7.2 containing 50 % Glycerol without preservatives.
    储存液
    Without preservative
    储存条件
    -20 °C
    储存方法
    Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    有效期
    12 months
  • 抗原
    Syntaxin 6 (STX6)
    别名
    Syntaxin 6 / STX6 (STX6 产品)
    别名
    CG7736 antibody, CT23545 antibody, Dm Syx6 antibody, DmSyx6 antibody, Dmel\\CG7736 antibody, SYN6 antibody, SYX6/10 antibody, Syx antibody, MGC78971 antibody, MGC132090 antibody, STX6 antibody, fb51b08 antibody, wu:fb51b08 antibody, zgc:110658 antibody, si:dkey-121a11.2 antibody, LOC734102 antibody, Syx6 antibody, Bmsyx6 antibody, Syntaxin-6 antibody, 2310039E05Rik antibody, 2410005I16Rik antibody, AA437559 antibody, AI415714 antibody, AI845861 antibody, Syntaxin 6 antibody, syntaxin 6 L homeolog antibody, syntaxin 6 antibody, Syx6 antibody, stx6.L antibody, STX6 antibody, stx6 antibody, Stx6 antibody
    背景
    Key requirements for protein transport are vesicular carriers with a full complement of machinery to enable them to find and fuse with the correct downstream compartment. This machinery includes the soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein (SNAP) receptors (SNAREs). SNAREs mediate diverse membrane fusion events such as neurotransmitter-filled vesicles fusing with the presynaptic plasma membrane. Syntaxin-6 is a q-SNARE found in endosomal transport vesicles. Syntaxin-6 has been shown by electron microscopy to localize mostly to the trans-Golgi network (TGN) and, to a lesser extent, to the Golgi stack.Synonyms: Golgi Marker, Syntaxin-6
    基因ID
    58244
    UniProt
    Q9JKK1
    途径
    Synaptic Vesicle Exocytosis
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