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- 抗原 See all Nemo-Like Kinase (NLK) 抗体
- Nemo-Like Kinase (NLK)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Nemo-Like Kinase antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 交叉反应
- 人
- 纯化方法
- Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
- 免疫原
- Purified His-tagged NLK protein was used to produced this monoclonal antibody.
- 克隆位点
- 6C1
- 亚型
- IgG2a
- Top Product
- Discover our top product NLK Primary Antibody
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anti-Nemo-Like Kinase (NLK) (AA 416-515) antibody
NLK 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B11 unconjugated
anti-Nemo-Like Kinase (NLK) antibodyNLK 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) antibodyNLK 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Nemo-Like Kinase (NLK) (AA 278-527) antibodyNLK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) (AA 278-527) antibodyNLK 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) (AA 1-515) antibodyNLK 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) (N-Term) antibodyNLK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nemo-Like Kinase (NLK) (AA 275-300) antibodyNLK 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB16621 unconjugated
anti-Nemo-Like Kinase (NLK) (AA 408-436), (C-Term) antibodyNLK 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB34651 unconjugated
anti-Nemo-Like Kinase (NLK) (Thr286) antibodyNLK 适用: 人 WB, FACS, IF/ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:300-5000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- Nemo-Like Kinase (NLK)
- 别名
- NLK (NLK 产品)
- 别名
- Amf antibody, Gpi1 antibody, AI194375 antibody, nlk antibody, nlkb antibody, zgc:111875 antibody, Xnlk antibody, nlk1 antibody, nlk2 antibody, NLK antibody, nemo-like antibody, RGD1561602 antibody, glucose-6-phosphate isomerase antibody, nemo like kinase antibody, nemo-like kinase, type 1 antibody, nemo-like kinase L homeolog antibody, Nemo-Like kinase antibody, nemo-like kinase antibody, Gpi antibody, NLK antibody, Nlk antibody, nlk1 antibody, nlk.L antibody, nlk antibody, nemo-like antibody
- 背景
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Synonyms: Serine/threonine-protein kinase NLK, Nemo-like kinase, Protein LAK1, NLK, LAK1
Background: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner (PubMed:25512613).
- 基因ID
- 51701
- UniProt
- Q9UBE8
- 途径
- Ubiquitin Proteasome Pathway
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