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CLNS1A 抗体

Name: CLNS1A 抗体
SKU: ABIN4903315
Availability: OutOfStock
Rating: 5 (1 reviews)

CLNS1A 适用: 人 WB, FACS, IHC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) antibody (ABIN4903315) (CLNS1A 抗体)

1 image

(1 validation)

产品编号 ABIN4903315

发货至: 中国

Datasheet as PDF

抗原 See all CLNS1A 抗体

CLNS1A (Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A))
适用
42

21

19

4

3

2

2

2

1

1

1

1

1

1
人
宿主
39

3
兔
Compatible Secondaries
克隆类型
39

3
多克隆
标记
22

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CLNS1A antibody is un-conjugated
应用范围
35

13

7

6

5

4

3

3

3

2

2

1
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

纯化方法

Affinity purification

免疫原

A synthetic Peptide of human CLNS1A

亚型

IgG

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anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (AA 148-176) antibody
CLNS1A 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19566 unconjugated

anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (C-Term) antibody
CLNS1A 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (C-Term) antibody
CLNS1A 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (AA 138-187) antibody
CLNS1A 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, Hamster, 猴, Bat, Pig, 小鸡 WB 宿主: 兔 Polyclonal unconjugated

anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (C-Term) antibody
CLNS1A 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) (C-Term) antibody
CLNS1A 适用: 人, 小鼠, 大鼠, 犬, 兔, Cow, 豚鼠, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated

anti-Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A) antibody
CLNS1A 适用: 人, 小鼠, 大鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500 - 1:1000
IHC 1:20 - 1:50
FC 1:20 - 1:50

限制

仅限研究用
生效 #102909 (Western Blotting)

by

AG Gruss, Institut für Genetik, Rheinische Friedrich-Wilhelms-Universität Bonn

No.

#102909

日期

2018.09.20

抗原

CLNS1A

Lot Number

03R181011

Method validated

Western Blotting

Positive Control

HeLa cells

Independent antibody

Negative Control

HeLa cells, CLNS1A knockdown using Dharmacon smartpool siRNA

Notes

Passed. ABIN4903315 specifically reveals endogenous CLNS1A in HeLa cell lysates while no signal is detected subsequently to knock-down of the antigen.

Validation Images

Western blot analysis of HeLa cell lysates without (HeLa) or with siRNA knock-down (HeLa/kd). CLNS1A was specifically detected using ABIN4903315 (A) and an independent antibody (B). Tubulin serves as loading control (C).
Full Methods
Primary Antibody

ABIN4903315

Secondary Antibody

HRP-conjugated goat anti-rabbit IgG (H+L) (Jackson ImmunoResearch, 111-035-003)

Full Protocol

Seed 3x10⁴ HeLa cells in DMEM (PAN Biotech, P04-41450, 2730618) supplemented with 10% FBS (PAN Biotech, P03-3302), without antibiotics, in 6 well plates in 2ml media.
Grow cells for 24h at 37°C and 5% CO₂ to 70% confluency.
Change medium.
Transfect cells with 60pmol ON-TARGETplus SmartPool siRNA human (Dharmacon, L-012571-00-0005, lot 170831) in 125µl 150mM NaCl added to 7.5µl Lipofectamine RNAiMax (13778030, lot 1911816) in 125µl 150mM NaCl.
Grow cells for 24h at 37°C and 5% CO₂.
Change medium.
Grow cells for 72h at 37°C and 5% CO₂.
Wash cells once with PBS (PAN Biotech, P04-36500, lot 3620618).
Detach cells with 100µl trypsin per well.
For WB the cells were once washed with PBS (PAN Biotech) before treated with 100µl of Trypsin (PAN Biotech, P10-023100, lot 6860417) per well.
Once cells are detached add 900µl medium.
Count cells.
Add 40µl 1x Laemmli buffer (Roth, K929.1, lot 227259243) to 2x10⁵ cells. Heat up to 99°C for 10min.
Separate 10µl of the lysates on a freshly cast denaturing 12% SDS-PAGE gel in an electrophoresis chamber for 1h at 120V.
Transfer proteins onto PVDF membrane (Merck, IPVH0010, lot K5KN7128I) with a wet blotting system for 1h at 80V.
Block the membrane with 1x Roti Block (Roth, A151.2, lot 148270222) for 30min at RT.
Incubation with primary
rabbit anti-CLNS1A antibody (antibodies-online, ABIN4903315, lot 03R181011) diluted 1:500 in 1x Roti Block for 1h at RT,
mouse anti-CLNS1A antibody (Santa Cruz, sc-271454, G3010) diluted 1:3000 in 1x Roti Block for 1h at RT, or
rat anti-tubulin antibody (YOL 1/34 gifted) diluted 1:1000 in 1x Roti Block for 1h at RT.
Wash membrane 2x for 10min with 1x TBST.
Incubation with secondary
HRP-conjugated goat anti-rabbit IgG (H+L) (Jackson ImmunoResearch, 111-035-003) diluted 1:1000 in 1x Roti Block for 1h at RT,
goat anti-mouse IgG (H+L) (Jackson ImmunoResearch, 115-005-003, lot 128525) diluted 1:10000 in PBS for 45min at RT, or
HRP-conjugated goat anti-rat IgG (H+L) (Jackson ImmunoResearch, 112-035-003) diluted 1:10000 in PBS for 45min at RT.
Wash membrane 5x for 5 min with TBS-T.
Reveal protein bands using ECL (GE Healthcare, RPN3244V2, 9633722) on an LAS mini system (GE healthcare).

Experimental Notes

Western blot using ABIN933127 on HeLa cell extracts reveals a band of the expected molecular weight. The same band is also detected by an independent CLNS1A antibody and disappears upon siRNA knock-down.
状态

Liquid

缓冲液

PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles.

储存条件

-20 °C

储存方法

Store at -20C. Avoid freeze / thaw cycles.
抗原

CLNS1A (Chloride Channel, Nucleotide-Sensitive, 1A (CLNS1A))

别名

CLNS1A (CLNS1A 产品)

别名

CLCI antibody, CLNS1B antibody, ICln antibody, 2610036D06Rik antibody, 2610100O04Rik antibody, Clci antibody, Clcni antibody, ICLN antibody, Icln antibody, pICln antibody, clci antibody, clns1b antibody, icln antibody, pIcIn antibody, chloride nucleotide-sensitive channel 1A antibody, chloride channel, nucleotide-sensitive, 1A antibody, chloride channel, nucleotide-sensitive, 1A L homeolog antibody, CLNS1A antibody, Clns1a antibody, clns1a.L antibody, clns1a antibody

背景

This gene encodes a protein that functions in multiple regulatory pathways. The encoded protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator. Pseudogenes of this gene are found on chromosomes 1, 4 and 6. Several transcript variants encoding different isoforms have been found for this gene.

分子量

26.215 kDa

基因ID

1207

UniProt

P54105

途径

Ribonucleoprotein Complex Subunit Organization