Luciferase 抗体
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- 抗原 See all Luciferase 抗体
- Luciferase
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适用
- Firefly
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Luciferase antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Luciferase or Luciferin 4-monooxygenase is a protein encoded by the luc gene which is produced in Photinus pyralis or the common eastern firefly.. Luciferase is localised to the peroxisome and is involved in chaperone binding. This protein falls under the ATP-dependent AMP-binding enzyme family and is one of the most extensively studied of the enzymes that catalyse light production in bioluminescent organisms and requires ATP and 02 as substrates. There are two reactions catalysed by luciferase, the first being the formation of an enzyme-bound luciferyl-adenylate and a second in which the luciferyl-adenylate undergoes an oxidative decarboxylation which results in the production of CO2, oxyluciferin, AMP, and green light with a wavelength of 562 nm. STJ97752 was developed from clone 6B8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of Luciferase.
- 特异性
- Luciferase Mouse Monoclonal Antibody (6B8) detects endogenous levels of Luciferase
- 产品特性
- Mouse Monoclonal to Luciferase.
- 纯化方法
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 免疫原
- Recombinant peptide derived from Luciferase
- 克隆位点
- 6B8
- 亚型
- IgG1
- Top Product
- Discover our top product Luciferase Primary Antibody
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- 应用备注
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WB 1:1000-2000
IHC 1:100-200 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- 抗原
- Luciferase
- Abstract
- Luciferase 产品
- UniProt
- P08659
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