CD58 抗体 (AA 29-215)
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- 抗原 See all CD58 抗体
- CD58
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抗原表位
- AA 29-215
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CD58 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Lymphocyte function-associated antigen 3(CD58) detection. Tested with WB, IHC-P, FCM in Human.
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Lymphocyte function-associated antigen 3(CD58) detection. Tested with WB, IHC-P, FCM in Human.
Gene Name: CD58 Molecule
Protein Name: Lymphocyte function-associated antigen 3 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- E. coli-derived human LFA3 recombinant protein (Position: F29-R215).
- 亚型
- IgG
- Top Product
- Discover our top product CD58 Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- CD58
- 别名
- CD58 (CD58 产品)
- 别名
- LFA-3 antibody, LFA3 antibody, ag3 antibody, CD58 molecule antibody, CD58 antibody
- 背景
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CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.
Synonyms: AG3 | CD58 | CD58 antigen | CD58 Molecule | LFA 3 | LFA-3 | LFA3 | P19256 - 基因ID
- 965
- UniProt
- P19256
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