CD27 抗体 (AA 20-191)
-
- 抗原 See all CD27 抗体
- CD27 (CD27 Molecule (CD27))
-
抗原表位
- AA 20-191
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This CD27 antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for CD27 antigen(CD27) detection. Tested with WB, IHC-P, FCM in Human.
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
-
Rabbit IgG polyclonal antibody for CD27 antigen(CD27) detection. Tested with WB, IHC-P, FCM in Human.
Gene Name: CD27 Molecule
Protein Name: CD27 antigen - 纯化方法
- Immunogen affinity purified.
- 免疫原
- E. coli-derived human CD27 recombinant protein (Position: A20-R191). Human CD27 shares 62.8% amino acid (aa) sequence identity with mouse CD27.
- 亚型
- IgG
- Top Product
- Discover our top product CD27 Primary Antibody
-
-
- 应用备注
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
-
- 抗原
- CD27 (CD27 Molecule (CD27))
- 别名
- CD27 (CD27 产品)
- 别名
- TNFRSF7 antibody, S152 antibody, Tnfrsf7 antibody, Tp55 antibody, S152. LPFS2 antibody, T14 antibody, CD27 molecule antibody, CD27 antigen antibody, CD27 antibody, Cd27 antibody
- 背景
-
CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Synonyms: CD 27 | CD27 | CD27 antigen | CD27 Molecule | CD27L receptor | LPFS2 | S152 | T14 | TNFRSF 7 | TNFRSF7 | TNFSF7 | Tp 55 | Tp55 | P26842 - 基因ID
- 939
- UniProt
- P26842
- 途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
-